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Fetal Bovine Serum for Stem Cell Culture

Fetal bovine serum (FBS) is one of the most widely used serum-supplements for in vitro cell culture. FBS contains a complex array of protein components, excellent cell growth factors, low endotoxin levels, and low hemoglobin concentrations, all of which are required by many cells to survive, grow, and divide. FBS from MP Bio offers excellent value for stem cell culture. With our quality assurance system from raw material to final product, we ensure reliable and consistent delivery of high quality FBS to our customers.

  • Tested on cell growth performance
  • Low endotoxin levels
  • Free of mycoplasma contamination
  • Specific virus testing (BVD, IBR,PI3)
  • Sterilization by triple 0.1-micron filtration
  • Country origin and traceability
SKU Product Name
0929168 CELLectTM FBS, GOLD, Heat Inactivated, US Origin
0929167 CELLectTM FBS, GOLD, US Origin
0929176 CellmaxxTM Fetal Bovine Serum, New Zealand Origin

FastGroTM, Fully Chemically Defined FBS Replacement for Stem Cell Culture

Due to its undefined nature, FBS can lead to unexpected differentiation of stem cells and biorisk of animal protein or pathogen contamination in FBS, such as bovine spongiform encephalopathy (BSE). To avoid these concerns, we offer FastGroTM, a fully chemically defined FBS replacement for stem cell culture use. It allows culturing a wide range of cells in vitro without the use of serum or any animal or human derived compound. Our FastGroTM ensures in cell culture:

  • Chemically defined nature without lot-to-lot variations
  • No animal or human derived materials or compounds
  • No interference with hormones or growth factors
  • Elimination of the risk of contaminants – viruses, mycoplasma, prions, etc.
  • Storage in the refrigerator, and no need for thawing before use
SKU Product Name
0926400 FastGro, Animal Free, Chemically Defined FBS Replacement

Attachment factors for successful stem cell culture

Attachment factors are critical for many adherent cell types in cell culture, including stem cells, and particularly when grown in serum-free culture. To facilitate cell adhesion, migration, growth, morphology, and differentiation, MP Bio offers a selection of attachment and matrix factors, such as collagen type I, fibronectin, and laminin.

SKU Product Name
021500268 Collagen Type I from calf skin
0219349220 Collagen Solution, 0.3%, 20 mL
0215112601 Fibronectin, human, >95%
0855913 Fibronectin, human, purified
0215002701 Laminin
02152690 Poly-L-lysine hydrochloride
02152689 Poly-L-Lysine hydrobromide
02150175 Poly-D-Lysine hydrobromide, MW 70,000-150,000
02102694 Poly-D-Lysine hydrobromide, MW 4,000-15,000

Chemically Defined Basal Media for Consistent Stem Cell Culture Results

Successful growth of stem cells requires high quality and reliable basal media, which contain amino acids, vitamins, inorganic salts, and a carbon source such as glucose. MP Biomedicals offers a complete range of products designed to support optimal stem cell growth, ensuring reproducible and consistent results.

Chemically defined powdered and liquid media are manufactured with minimal risk of contamination in an environmentally controlled facility. Chemicals used in production meet the highest quality and purity standards. The result is high cell yield and a homogeneous cell population for research and manufacturing applications.

  • Chemically defined essential components
  • Lot-to-lot consistency
  • Extremely low endotoxin levels
  • Free of mycoplasma contamination
  • Animal-component free
  • Liquid and powder formats
SKU Product Name
091233254 DMEM (1X), without L-glutamine
091233354 DMEM (1X), with 4.5 g/L dextrose, with L-glutamine
091642149 DMEM (1X), with 4.5 g/L dextrose, without L-glutamine and L-leucine and sodium pyruvate
091642954 DMEM (1X), with 4.5 g/L glucose, without L-glutamine and inositol
0912334 DMEM (1X) with 20 mM HEPES, without L-glutamine or sodium bicarbonate
09103312 DMEM, with L-glutamine, with 4.5 g/L glucose, without sodium bicarbonate, powder
091210254 MEM (1X), without L-glutamine
091641454 MEM (1X), Earle's Salts and 2.0 g/L sodium bicarbonate, without L-glutamine, L-cysteine, L-cystine, and L-methionine
091601149 MEM, Amino Acids (50X) solution
09101012 MEM, Earle's Salts and L-glutamine, without sodium bicarbonate, powder
091646454 RPMI (1X), without L-glutamine, L-cysteine, L-cystine, and L-methionine
091646854 RPMI 1640 with 2 g/L sodium bicarbonate, without L-glutamine and glucose, powder
091260254 RPMI 1640 (1X), without glutamine
0912603 RPMI 1640 (1X), with L-glutamine and sodium bicarbonate
091440049 Ham's F-10 (10X), without L-glutamine and sodium bicarbonate, with 12.4 mg/L phenol red
09104012 Ham's F-10, with L-glutamine, without sodium bicarbonate, powder
09104212 Ham's F-12 (modified) with L-glutamine, without sodium bicarbonate, powder
091242354 Ham's F12 (1X), with L-glutamine
091960454 Dulbecco's Phosphate Buffered Saline (10X), without calcium and magnesium
0918604 Dulbecco's Phosphate Buffered Saline (1X), without calcium and magnesium

Bovine Extract for Maximum Mitogenicity in Stem Cell Culture

Containing a full spectrum of putative mitogens and growth factors, bovine pituitary extract (BPE) and bovine brain extract (BBE) continue to be the most successful tissue-sourced extracts for culturing a wide range of cells in vitro, particularly stem cells. To maintain the full spectrum of biological activities and minimize biorisk of animal origin products, MP Bio recently launched BPE and BBE made from young, healthy New Zealand cattle with USDA approved sources.

  • Promotes maximum cell mitogenicity
  • Promotes differentiation of pluripotent stem cells
  • Sterile filtration from non-lyophilized tissue
  • Maintains consistent activity in serum-free culture systems
SKU Product Name
092850450 Bovine Pituitary Extract (2.5 mL)
092850445 Bovine Pituitary Extract (10 mL)
092850550 Bovine Brain Extract

Bovine Extract for Maximum Mitogenicity in Stem Cell Culture

One primary function of BSA as an additive is to bind, protect, and stabilize a range of important small molecules and ions in the cell culture media, especially serum-free media. To maximize its performance in stem cell culture, BSA from MP Biomedicals – New Zealand (AlbumiNZTM) has been purified by chromatography to ensure the highest purity. Unlike with other extraction procedures (e.g. Cohn, Heat Shock), our bovine serum albumin is more biologically intact and highly retains the cell growth stimulating activity.

  • Superior cell culture performance over Cohn's Albumin Fraction V
  • Intact proteins with high activity
  • Highest purity and consistency
  • New Zealand sourced raw materials having negligible BSE risk
  • Free of BSE and List A animal diseases
SKU Product Name
02199899 AlbumiNZTM Low Fatty Acid BSA
02199896 AlbumiNZTM Low Endo Acid BSA

Cell Detachment

For adherent stem cell cultures, cells first need to be detached before further study. This detachment process is commonly performed with a mixture of trypsin-EDTA. In some instances, trypsin reduces cell viability due to damage to the membrane and extracellular matrix. As a superior alternative to trypsin, we recommend Accutase®, a blend of proteolytic and collagenolytic enzymes for gentle cell detachment:

  • Gentle and efficient for cell detachment
  • Maximum cell viability compared to trypsin
  • Proven effective over a wide range of primary cells
  • Versatile on various cell culture surfaces
SKU Product Name
091000449 Accutase® Cell Detachment Solution
091689649 Trypsin-EDTA solution

Detect and Eliminate Mycoplasma Contamination in Stem Cell Cultures

One of the major issues in dealing with stem cell cultures is mycoplasma infection. The presence of mycoplasma can alter a variety of cellular characteristics and functionalities. Therefore, a fast and simple mycoplasma detection method is necessary to ensure the reliability, reproducibility, and consistency of your stem cell research.

MP Bio is pleased to announce the launch of Myco-Sniff-Rapid™ Mycoplasma Luciferase Detection Kit, ensuring:

  • Rapid results in 20 minutes
  • Sensitive and specific for detecting all common mycoplasma species
  • No interference with cell culture media components
  • Simple operation: add reagents directly to the culture supernatant

If quantification and species determination of mycoplasma are required, we recommend our Myco-Sniff-Valid™ Mycoplasma PCR Detection Kit, which can sensitively detect mycoplasma as low as 10 CFU/mL, as well as the species.

Related products:

SKU Product Name
09305040 Myco-Sniff-Rapid Mycoplasma Luciferase Detection Kit
093050201 Myco-Sniff Mycoplasma PCR Detection Kit
093050301 Myco-Sniff-Valid Mycoplasma PCR Detection Kit
093050044 Mycoplasma Removal Agent (MRA)

Cell Culture Antibiotics: Keep Your Stem Cell Cultures Contamination Free

High quality antibiotics from MP Bio eliminate cell culture contamination with minimal impact on stem cells. These easy-to-use antibiotics can efficiently keep cell cultures contamination-free from a wide range of microbiological contaminants.

SKU Product Name
0916723 Amphotericin B, 250 μg/mL (Fungizone)
091676045 Gentamicin Sulfate Solution, 10 mg/mL
0916762 Gentamicin Sulfate Solution, 50 mg/mL
09167254 G418 Sulfate, 50 mg/mL (Geneticin)
091672048 Kanamycin Sulfate, 5 mg/mL
091670249 Penicillin-Streptomycin (10,000 IU/mL, 10 mg/mL)
091674049 Penicllin-Streptomycin-Amphotericin B (100X)

Stem Cell Cryopreservation

With over 40 years of experience manufacturing and supplying cell biology products, MP Bio understands the challenges associated with stem cell cryopreservation. Cryopreservation media from MP Bio ensures:

  • Consistent high cell viability
  • Serum-free and protein-free formulation
  • Validation with multiple cell types
  • Long-term cell storage
  • Balanced components for maintaining cellular functions
  • Long shelf life
SKU Product Name
092030346 pZerve Cryopreservation Solution, 20 mL
0920303M2 pZerve Cryopreservation Solution, 60 mL
092780248 Cell Cryopreservation Medium with 10% DMSO, 50 mL
092780145 CryopresTM Dimethyl Sulfoxide (>99.9% USP DMSO), 10 mL
092780148 CryopresTM Dimethyl Sulfoxide (>99.9% USP DMSO), 50 mL
092780349 2-8 CELLsiumTM medium for short-term cell storage, 100 mL
092780354 2-8 CELLsiumTM medium for short-term cell storage, 500 mL