Blotting and Hybridization

The Vacuum Blotter for quick and efficient transfer of DNA or RNA onto suitable membrane such as Positive Nylon membrane. Transfer efficiency close to 100% in less than one hour with low transfert solution consuption. Largworking area 28 x 28 cm.

The intrinsic positive charge of this membrane ensures higher sensitivity and superior results even after multiple reprobing. The membrane keeps its positive charge from pH 3 to pH 12.

The intrinsic positive charge of this membrane ensures higher sensitivity and superior results even after multiple reprobing. The membrane keeps its positive charge from pH 3 to pH 12.

Sufficient for stripping 20-40 mini blots.Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or

Masks are used to customize the blotter's working area to the size of your gel.

20xSSC solution for use in Southern and northern blotting.

Biotrans PVDF membranes are naturally hydrophobic polyvinylidene difluoride membrane developed for use in protein transfer and immobilization procedures. The Biotrans PVDF membrane is a pure white microporous solid phase support which undergoes strong hydrophobic interaction with a wide range of proteins. Immobilized proteins can be used directly for protein .Immobilized proteins can be used directly for protein sequencing or amino acid analysis, and can be visually detected with all common staining reagents, including coomassie blue, amido black, ponceau S, and colloidal gold.

BIOTRANS (+) has a pore surface populated by a high density of quarterly ammonium groups which make it strongly cationic. The positive surface charge is maintained in the range pH 3 to over pH 10 and promotes strong ionic binding of negatively charged proteins and nucleic acids. BIOTRANS (+) membrane is ideally suited to new rapid transfer techniques for nucleic acid which provide excellent levels of sensitivity (e.g. Alkaline Transfer Procedure ). In addition, the membrane's immediate immobilization characteristics make it suitable for prolonged transfer procedures without the risk of nucleic acid diffusion from the membrane.

Hybridization solution containing 50% formamide and 2X SSC.