NEW Product: MagBeads FastDNA™ Kit for Feces request your FREE sample
How DSS induces physiological responses that mimic IBD
Chemically induced murine models of intestinal inflammation, such as DSS-induced colitis models, are widely used because they are easy to induce and the onset, duration and severity of inflammation are immediate and controllable. By titrating the amount of DSS up or down, you can model severe to mild inflammatory disease.
DSS works by direct and selective toxicity to epithelial cells. DSS causes a loss of surface epithelium, enabling luminal microbes and their metabolites enter the lamina propria and induce an inflammatory response (Figure 1).Figure 1. DSS is directly toxic to epithelial cells, causing loss of the surface layer of epithelial cells, enabling gut microorganisms and their products to enter the lamina propria, and stimulating an inflammatory response.
The advantages of DSS-induced models
The DSS-induced colitis model has some advantages when compared to other animal models of colitis. As mentioned above, an acute, chronic, or relapsing model can easily be generated by changing the concentration and administration cycle of DSS in rats and mice. Moreover, dysplasia that resembles the clinical course of human ulcerative colitis (UC) occurs frequently in the chronic phase of DSS-induced colitis.
Studies validating DSS models using various therapeutic agents for human IBD have shown that DSS-induced colitis can be used as a relevant model for the translation of mice data to human disease.
For more on DSS models of colitis, we recommend the review:
Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis
Derrick D Eichele and Kusum K Kharbanda. World J Gastroenterol. 2017 Sep 7; 23(33): 6016–6029. PMCID: PMC5597494.
Creating an IBD model
The protocol for generating a DSS-induced colitis model is simple to implement—simply dissolve the DSS powder in autoclaved drinking water to create a solution of the correct dose. The duration of the treatment will depend on the strain of rodent as well as how you choose to define your model based on your research aims.
Dextran Sodium Sulfate, Colitis Grade
Our DSS is the most widely cited product on the market for generating DSS-induced colitis modelsView Product
Verifying your IBD model
A successful IBD model can be verified quickly and easily using standard laboratory procedures
- The body mass of treated mice will drop rapidly over the course of seven days.
- At the tissue level, histological examination of the colon by Hematoxylin & Eosin staining on will show gross structural changes, including the destruction of the epithelial lining.
Getting the most out of your DSS colitis model
MP Biomedicals delivers sample preparation solutions to help you with the downstream analysis of your DSS colitis model. Our diverse collection of products ensure you can find reliable and easy-to-use tools to enhance your experiments.
Our FastPrep systems can be used to efficiently isolate DNA, RNA, protein, and lipids from your tissues, allowing you to uncover the molecular mechanisms of interest in your DSS-induced colitis model. FastPrep is suitable for use with a variety of sample types, including fecal matter and tissue biopsies.
Our FastPrep systems allow you to extract biological materials from even the most challenging samples in under 30 minutesView Product
Feeding your animals
An important feature of a successful IBD model is the accompanying diet. MP Biomedicals offers a complete range of high quality and customizable rodent diets and supplements to suit any research needs, including:
- High-fat diets
- Low-fat diets
- High carbohydrate diets
- Choline deficient diets
Animal Diets and Feed
Explore our high-quality animal research dietsView Product