Protocatechuate-3,4-Dioxygenase
Application Notes
Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into ß-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used for enzymatic determination of choline esterase
| SKU | 0215197525 |
| Application Notes | Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into ß-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay. |
| Base Catalog Number | 15197525 |
| CAS # | 9029-47-4 |
| Format | Powder |
| Pack Size | 25 U |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Source | Pseudomonas sp. |
| Specific Activity | ≥3 u/mg solid |
| Unit Definition | One unit will oxidize one µmole of protocatechuate to 3-carboxy-cis,cis-muconate per minute at pH 7.5 at 37 °C. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [02151975] lot ID: U1122264139](https://www.mpbio.com/static/version1742546773/frontend/Mpbio/default/en_US/images/document.png "MP COA [02151975] lot ID: U1122264139")