CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.
Application Notes
CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Detergent for solubilizing membrane proteins
| SKU | 02190319-CF |
| Aggregation Number | 4-14 (0-0.1 M Na+) |
| Alternate Names | 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate; N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] amino]-1-propanaminium inner salt |
| Application Notes | CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors. |
| Base Catalog Number | 190319 |
| CAS # | 75621-03-3 |
| Critical Micelle Concentration | 6-10 mM |
| Hazard Statements | H315-H319-H335-H360D |
| Melting Point | 265°C (Lit.) |
| Molecular Formula | C32H58N2O7S |
| Molecular Weight | 614.9 |
| pH | 4.0 - 7.0 (10% aq soln) |
| Purity | ≥98% |
| Safety Symbol | GHS07, GHS08 |
| Solubility | Soluble in water (100 mg/mL - clear, colorless to slightly yellow solution). |
| Typical Working Concentration | Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [02190319-CF] lot ID: YC10013](https://www.mpbio.com/static/version1780565991/frontend/Mpbio/luma/en_US/images/document.png "MP COA [02190319-CF] lot ID: YC10013")
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