Guanidine Hydrochloride
Application Notes
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Strong denaturant
| SKU | 02101905-CF |
| Alternate Names | Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride; Guanidium chloride; Guanidine HCl |
| Application Notes | Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. |
| Base Catalog Number | 101905 |
| Beilstein Registry Number | 3591990 |
| CAS # | 50-01-1 |
| Density | 1.3 g/cm³ |
| EC Number | 200-002-3 |
| Format | Powder |
| Grade | Practical Grade |
| Hazard Statements | H302-H315-H319 |
| Melting Point | 178-185°C |
| Molecular Formula | CH5N3·HCl |
| Molecular Weight | 95.53 g/mol |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| RTECS Number | MF4300000 |
| Safety Symbol | GHS07 |
| Solubility | in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, g/100ml at 20°C: 215 |
| Typical Working Concentration | Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [101905] lot ID: M3529](https://www.mpbio.com/static/version1742546773/frontend/Mpbio/default/en_US/images/document.png "MP COA [101905] lot ID: M3529")
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