DOB 2% Galactose, 1% Raffinose allows for preparation of a custom synthetic defined yeast media and includes a complete yeast nitrogen base (YNB) composed of buffering salts, vitamins and trace elements, with Galactose and Raffinose as the carbon source and Agar.
Application Notes
DOBA is a complete yeast nitrogen base with a carbon source and agar. A complete supplemented synthetic defined medium is easily made by mixing DOBA and CSM, a complete supplement mixture containing amino acids, adenine and uracil.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
| SKU | 114026912-CF |
| Alternate Names | Dropout Base with Agar |S.cerevisiae Growth medium with Agar |
| Application Notes | DOBA is a complete yeast nitrogen base with a carbon source and agar. A complete supplemented synthetic defined medium is easily made by mixing DOBA and CSM, a complete supplement mixture containing amino acids, adenine and uracil. |
| Base Catalog Number | 4026912 |
| Format | Powder |
| Formulation Details | Components :(Per liter medium) Nitrogen Sources : Ammonium Sulfate 5 g Carbon Source: Galactose 20 g Raffinose 10 gVitamins : Biotin 0.002 mg; Calcium Pantothenate 0.4 mg; Folic Acid 0.002 mg; Inositol 2 mg; Niacin 0.4 mg; P-Aminobenzoic Acid 0.2 mg; Pyridoxine Hydrochloride 0.4 mg; Riboflavin 0.2 mg; Thiamine Hydrochloride 0.4 mg Trace Elements: Boric Acid 0.5 mg; Copper Sulfate 0.04 mg; Potassium Iodide 0.1 mg; Ferric Chloride 0.2 mg; Manganese Sulfate 0.4 mg; Sodium Molybdate 0.2 mg; Zinc Sulfate 0.4 mg Salts: Potassium Phosphate; Monobasic 1000 mg; Magnesium Sulfate 500 mg; Sodium Chloride 100 mg; Calcium Chloride 100 mg Agar 17 g |
| Grade | Molecular Biology Certifiedâ„¢ |
| Media Type | Yeast Dropout Base (DOB) |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice |
| pH | 6.0 ± 0.3 |
| Preparation Method | Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in DOB formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution. |
| Solubility | Soluble in Water (53.7 g/L) |
| Typical Working Concentration | 53.7g/liter |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
