Spermine is a naturally occuring polyamine that occurs in all eukaryotes, but is rare in prokaryotes. It is essential for cell growth in both normal and neoplastic tissue. Spermine is formed through the addition of a aminopropyl group to spermidine by spermine synthase. Spermine is strongly basic in character, and in aqueous solution at physiological pH, all of its amino groups will be positively charged.

Application Notes

Used to precipitate DNA from low salt aqueous buffers. Spermine tetrahydrochloride was used to precipitate DNA for flow cytometric analysis from fresh frozen breast cancer tissue, archival tissue of salivary gland, renal and thyroid tumors and red blood cells of chicken.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Isolation of DNA | Precipitation of DNA

SKU	02100474-CF
Alternate Names	N,N-Bis(3-aminopropyl)-1,4-butanediamine tetrahydrochloride; Geontine Tetrahydrochloride
Application Notes	Used to precipitate DNA from low salt aqueous buffers. Spermine tetrahydrochloride was used to precipitate DNA for flow cytometric analysis from fresh frozen breast cancer tissue, archival tissue of salivary gland, renal and thyroid tumors and red blood cells of chicken.
Base Catalog Number	100474
Beilstein Registry Number	3911771
Biochemical Physiological Actions	Mixed NMDA agonist/antagonist at the polyamine site. Neuroprotective effects have been observed at high concentrations (1 mM), while neurotoxicity is observed at lower concentrations. It enhances agonist effectiveness at the strychnine-insensitive glycine site.
CAS #	306-67-2
EC Number	206-189-8
Format	Powder
Hazard Statements	H315-H319
Melting Point	312-314 °C (Lit.)
Molecular Formula	C10H26N4 · 4HCl
Molecular Weight	348.2
Personal Protective Equipment	Dust Mask, Eyeshields, Gloves.
pH	6.6 (1M)
Purity	≥96%
RTECS Number	EJ7230000
Safety Symbol	GHS07
Solubility	Soluble in water (100 mg/mL - clear, colorless solution).
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.