Cloning & Transformation

This yeast transformation kit provides the reagents needed to perform an easy and quick transformation of yeast cells. Can be used to transform linear DNA into P. pastoris for protein expression studies.

Transformation kit optimized for rapid and user-friendly preparation, transformation and storage of competent bacterial cells.

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a β-galactosidase substrate. In the presence of β-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate.

Ampicillin Sodium Salt

IPTG (Isopropyl-ß-D-thiogalactopyranoside) is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli.

Kit designed for high-throughput transformation of yeast cells with efficiencies up to 10³ transformants per μg plasmid DNA.