Blotting and Hybridization Buffers

Sufficient for stripping 20-40 mini blots.Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or

3 M trisodium citrate solution for use in Southern and northern blotting.

20xSSC solution for use in Southern and northern blotting.

Hybridization solution containing 50% formamide and 2X SSC.

Hybridization solution containing 50% formamide and 2X SSC.

Deionized Formamide for use in molecular biology and as a denaturing agent for nucleic acids in gel electrophoresis or hybridization experiments.