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Preparation of Protoplasts

Pectolyase Y-23 (A. japonicus)

Pectolyase Y-23 (A. japonicus)

Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases.

Unit Definition: One unit is defined as an increase in A235 by 1.0 in the reaction mixture per minute.

Pectolyase Y-23 (A. niger)

Pectolyase Y-23 (A. niger)

Highly purified enzyme from Aspergillus niger used for isolation of biologically active protoplasts from a wide spectrum of higher plants and tissues.

Cellulase, >16,000 Units/g

Cellulase Y-C (from Trichoderma viride), 10 g

Cellulase Y-C enzyme retains very high filter paper decomposing activity and showed an appreciable amount of hemicellulase. Actually, this enzyme removed cell walls from plant tissues in shorter incubation period without loss of biological activity of the materials.

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