Applications of NZ bovine proteins
- Thrombin finds applications in a variety of coagulation assays, clotting factor tests and defibrination of blood or plasma for serum controls, and in clot-activated blood collection tubes
- Fibrinogen can be used for preparation of Fibrin plates for analysis of fibrinolytic enzymes, as a substrate for clotting assays, and in the study of Fibrinogen degradation products
- Broad range of AlbumiNZ brand of BSA are available as blocking reagents.
- IgG is a preferred blocking agent in certain assays due to its higher MW over BSA and that it could help reduce cross reactivity of the molecule
- Selection of the right product would depend on the analyte being used
- Various grades of BSA are available for use in mammalian cell culture, stem cell research, monoclonal Ab and vaccine manufacture
- Chromatographically extracted BSA have clear advantages over BSA from other manufacturing processes as detailed in the brochure
MP Biomedicals New Zealand Limited
About MP Biomedicals New Zealand
MP Bio New Zealand Proteins are Chromatographically Extracted from Bovine Plasma, under Quality Systems that are ISO Certified and Audited to cGMP Principles.
Advantages of MP Bio’s chromatographic extraction process
1. MP Bio NZ BSA outperforms other BSA in Cell Culture
2. Uncompromised reliability, increased productivity
3. High lipid content compared to competition
MP Biomedicals New Zealand bovine proteins are chromatographically extracted under quality systems that are ISO 9001 certified and audited to cGMP principles.
MP Biomedicals chromatographically purified bovine AlbumiNZ has demonstrated superior growth rates in cell culture (CHO and SP2/0 cells) compared with traditional Cohn and Heat Shock BSA.
Graphs depict a comparison in quality, yield and productivity using MP Biomedicals Low IgG BSA versus an industry leading competitor BSA.
The scale of the bioreactor was 10,000 L and the processed cell line was a murine myeloma NS0.
This evaluation was done by a prominent worldwide biopharma company.
“Chromatographically purified NZ BSA from MP Biomedicals has been tested successfully with parasite infected cell lines representing all major endemic continents. Additionally, DNA transfections using various selectable agents (WR99210, blasticidin S, trimethoprim) have shown to be successful and it is also compatible with the c-SNARF limiting dilution assay.”
“The use of MP Bio-New Zealand BSA during IVF and IVC resulted in more and better quality embryos than a competitor BSA”
(Schumann, Theriogenology (2002) 57:1:527)
Higher quality compared to Heat Shock BSA
- Higher monomer to dimer ratio in the chromatographically purified AlbumiNZ BSA
- Fewer bands on SDS-Page in the chromatographically purified AlbumiNZ BSA indicating a higher purity of the BSA
Lane 1: Heat Shock BSA
Lane 2: AlbumiNZ™ Low Free Fatty Acid BSA
Lane 3: AlbumiNZ™ Low IgG BSA
Lane 4: AlbumiNZ™ Low Endotoxin BSA
Lane 5: AlbumiNZ™ Microbiological Grade BSA
Lane 6: MW Standards (kDA listed)
Higher Lipid content compared to competitor BSA
- Except for the low free fatty acid BSA, all other MP Biomedicals New Zealand BSA grades returned a lipid content of 35 mg/g of BSA when tested by an independent laboratory. This is 2.5x higher than the lipid content in competitor BSA produced from a chromatographic and heat shock process.
- This high lipid content can benefit cell culture researchers by way of reducing the need to add components such as cholesterol concentrate or other additives and could lead to significantly higher titer values at scale