Western Blot Workflow
Protein Extraction & Loading >Ships in One Business Day
From RIPA Lysis Buffer to Fluorescent Loading Buffers, our portfolio of high-quality reagents and buffers are ideal for initial protein extraction and PAGE loading. Combine with MP Bio’s loading controls and epitope tag antibodies to accurately quantify protein expression levels and verify the specificity of a protein of interest for Western blot analysis. Skip the gel staining step and visualize proteins post-electrophoresis with our Quick Fluorescent Loading Buffers and Fluorescent Protein Marker.
Category | Product | Cat. No. |
RIPA Lysis Buffer | 04822792 | |
Protein Quantification | 08W00021 | |
Inhibitor Cocktails | 08W00017 | |
08W00018 | ||
Loading Buffers | 08W00013 | |
08W00014 | ||
08W00015 | ||
08W00016 | ||
Protein Ladders | 08W00009 | |
08L100025-CF | ||
08W00027 |
PAGE Analysis >Ships in One Business Day
MP Bio now offers a comprehensive collection of pre-cast tris-glycine and bis-tris gels compatible with Bio Rad's Mini Protean Tanks, as well as running buffers for high-resolution protein separation. Achieve rapid electrophoresis with our Ultra-Fast Running Buffer, designed to shorten run times to about 20 minutes. Improve the overall experimental efficiency of electrophoresis using a combination of our precast gels, Quick Fluorescent Loading Buffer, Fluorescent Protein Marker, and Ultra-Fast Running Buffers.
Category | Product | Cat. No. |
Running Buffers | 08W00010 | |
08W00011 | ||
08W00012 | ||
08W00025 | ||
08W00026 | ||
Precast Gels | 08W00001 | |
08W00002 | ||
08W00003 | ||
08W00004 | ||
08W00005 | ||
08W00006 | ||
08W00007 | ||
08W00008 | ||
08W00022 | ||
08W00023 | ||
08W00024 | ||
Gel Staining Solution | 08X10001 |
Western Blot Reagents >Ships in One Business Day
Our Western blot assay reagents include solutions to simplify the process and improve results. Western Stripping Buffer enables the reuse of membranes for multiple probing, saving both time and cost. In addition, OneStep Western Blocker and Signal Enhancer provides blocking and primary and secondary antibody hybridization in one step, while also enhancing signal development with HRP or AP substrates. This three-in-one solution is an ideal alternative for researchers seeking to streamline the Western blot procedure and minimize laborious steps.
Category | Product | Cat. No. |
Blocker and Signal Enhancer | 08X100002 | |
Membrane Wash | 08W00020 | |
ECL Substrate | 08X100003 | |
WB Stripping | 08W00019 |
Resources

Triple Color Precision Protein Ladder (10 - 180 kDa)
Ready to use three color protein standard with 10 pre-stained proteins for monitoring protein separation
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Ultra-Blue Fast PAGE Gel Staining Solution
A ready to use protein staining solution for SDS-PAGE gels that enhances detection and eliminates the need for destaining
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OneStep Western Blocker and Signal Enhancer (Protein-Free)
Provides blocking and primary and secondary hybridization in one step while enhancing signal development with HRP or AP substrates.
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Ultra-Sense Femto-Plus Western ECL Substrate
Our Luminol-based substrate is designed for HRP-conjugated primary & secondary antibodies to detect target proteins that are too small for typical ECL substrates.
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