SPINeasy Virus RNA kit is a silica-membrane spin-column kit that enables quick and convenient extraction of virus RNA from cell culture media and bodily fluids such as saliva and serum, without the use of toxic substances such as phenol and chloroform. Through a simple workflow, virus RNA is typically extracted within 20 minutes and is immediately available for downstream applications such as RT-PCR and RT-qPCR.
Kit performance
The following results are RT-PCR of RNA extracted from various virus-spiked samples using SPINeasy Virus RNA Kit.
Figure 1: RT-PCR amplification of a viral-specific gene from RNA extracted from the indicated samples spiked with Influenza B virus, using SPINeasy Virus RNA Kit. M: DNA marker; Lane 1: PBS; Lane 2: DMEM, serum-free; Lane 3: DMEM with 10% FBS; Lane 4: Virus Transport Medium; Lane 5: Saliva; Lane 6: Serum; Lane 7: Negative Control.
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Application Notes
The SPINeasy Virus RNA Kit quickly and efficiently isolates high-quality, total RNA from cell culture media and bodily fluids in approximately 15 minutes using and SPIN columns for the purification process without any toxic chemicals.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
RNA extracted from SPINeasy Virus RNA Kit can be used in several downstream applications, including RT-PCR, qPCR, RNA-Seq,, and Northern Blot Analysis, Genomics, Molecular Biology
| SKU | 116537050 |
| Alternate Names | SPINeasy RNA Extraction Kit for Virus; Total RNA Viral Extraction Kit |
| Application Notes | The SPINeasy Virus RNA Kit quickly and efficiently isolates high-quality, total RNA from cell culture media and bodily fluids in approximately 15 minutes using and SPIN columns for the purification process without any toxic chemicals. |
| Base Catalog Number | 65370 |
| Product Line | SPINeasy |
| Components | 30 mL Lysis Buffer VR, 6 mL Wash Buffer VR, 10 mL Nuclease-free water, 1 vial Carrier RNA, 50 x Column VR with collection tubes |
| Pack Size | 50 Preps |
| Sample Type | Cell culture media and bodily fluids including serum and saliva; 100 - 500 µL of sample |
| Storage | Store at Room Temperature (15-30 °C), in a dry environment |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [116537050] lot ID: FC050A](https://www.mpbio.com/static/version1744790984/frontend/Mpbio/default/en_US/images/document.png "MP COA [116537050] lot ID: FC050A")