SPINeasy RNA Kit for Tissue is a silica-membrane spin-column kit that enables quick and convenient purification of total RNA from various animal tissues, plant tissues and tissue cultures. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrepĀ® instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Purified RNA is of high quality and integrity and immediately ready for RT-PCR and other downstream applications.
Kit performance
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Figure 1: RNA extracted from various samples using SPINeasy RNA Kit for Tissue, analyzed using agarose gel electrophoresis. M: DNA marker; Lane 1: Liver; Lane 2: Spleen; Lane 3: Kidney; Lane 4: Lung; Lane 5: Heart; Lane 6: Ginkgo biloba leaf; Lane 7: Prunus davidiana leaf; Lane 8: Cherry leaf; Lane 9: Begonia leaf; Lane 10: Tomato leaf; Lane 11: Peanut leaf; Lane 12: Potato leaf.
Figure 2: RT-PCR amplification of RNA extracted from various samples using SPINeasy RNA Kit for Tissue. M: DNA marker; Lane 1: Liver; Lane 2: Spleen; Lane 3: Kidney; Lane 4: Heart; Lane 5: Lung; Lane 6: Negative control (rabbit Ī²-actin); Lane 7: Ginkgo biloba leaf; Lane 8: Prunus davidiana leaf; Lane 9: Cherry leaf; Lane 10: Begonia leaf; Lane 11: Tomato leaf; Lane 12: Peanut leaf; Lane 13: Potato leaf; Lane 14: Negative control (plant 18S).
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Application Notes
The SPINeasy RNA Kit for Tissue quickly and efficiently isolates high-quality, total RNA from various animal tissues, plant tissues and tissue cultures in approximately 15minutes using a specialized Lysing Matrix A for cell lysis and SPIN columns for the purification process.
Usage Statement
Research Use Only (RUO)
Key Applications
Total RNA extracted from SPINeasy RNA Kit for Tissue can be used in several downstream applications, including RT-PCR, qPCR, Sequencing (NGS), and Northern Blot Analysis, Genomics, Molecular Biology
| SKU | 116543050 |
| Alternate Names | SPINeasy RNA Extraction Kit for Tissues, Total RNA Extraction Kit for Tissues |
| Application Notes | The SPINeasy RNA Kit for Tissue quickly and efficiently isolates high-quality, total RNA from various animal tissues, plant tissues and tissue cultures in approximately 15minutes using a specialized Lysing Matrix A for cell lysis and SPIN columns for the purification process. |
| Base Catalog Number | 6535050 |
| Product Line | SPINeasy |
| Components | 50x Lysing Matrix A, 60 mL Lysis Buffer R, 12 mL Wash Buffer R, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 50x Column R with collection tube |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | 10 - 30 mg Animal Tissue; 50-100 mg Plant Tissue; 1x107 Cultured Cells |
| Storage | Store at Room Temperature (15-25 Ā°C) in a dry environment |
| Usage Statement | Research Use Only (RUO) |
