SPINeasy RNA Kit for Bacteria is a silica-membrane spin-column kit that enables quick and convenient purification of total RNA from gram-positive and gram-negative bacteria. Included in the kit is our specially formulated RNASS solution that stabilizes and protects RNA in bacteria samples. The use of Lysis Buffer R and Lysing Matrix B in combination with FastPrepĀ® instruments from MP Biomedicals enables highly efficient lysis of bacterial samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Total RNA of high quality and integrity can be purified typically within 40 minutes and is immediately available for RT-PCR and other downstream applications.
Kit performance
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Figure 1: Total RNA extracted from bacteria samples using SPINeasy RNA Kit for Bacteria, analyzed using agarose gel electrophoresis. M: DNA marker; Lane 1: E. coli; Lane 2: S. aureus.
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Figure 2: RT-PCR amplification of RNA extracted from bacteria samples using SPINeasy RNA Kit for Bacteria. M: DNA marker; Lane 1: E. coli; Lane 2: S. aureus; Lane 3: Negative control.
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Application Notes
The SPINeasy RNA Kit for Bacteria quickly and efficiently isolates high-quality, total RNA from G+ and G- bacteria using a specialized Lysing Matrix B for cell lysis and SPIN columns for the purification process.
Usage Statement
Research Use Only (RUO).
Key Applications
Total RNA extracted from SPINeasy RNA Kit for Bacteria can be used in several downstream applications, including RT-PCR, qPCR, Sequencing (NGS), Microbial transcriptomics, RNA-Seq, Northern Blot Analysis, Genomics, Molecular Biology
| SKU | 116541050 |
| Alternate Names | RNA Extraction Kit for Microbes; Total RNA Extraction Kit for Bacteria and Fungi |
| Application Notes | The SPINeasy RNA Kit for Bacteria quickly and efficiently isolates high-quality, total RNA from G+ and G- bacteria using a specialized Lysing Matrix B for cell lysis and SPIN columns for the purification process. |
| Base Catalog Number | 65410 |
| Product Line | SPINeasy |
| Components | 50x Lysing Matrix B, 15 mL RNASS, 60 mL Lysis Buffer R, 6 mL Wash Buffer R, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 50x Column R with collection tube |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Bacteria (Gram +/-), Fungi, Yeast |
| Storage | Store at Room Temperature (15-25 Ā°C) in a dry environment |
| Usage Statement | Research Use Only (RUO). |
