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SPINeasy® DNA/RNA/Protein All-In-One Kit

SPINeasy® DNA/RNA/Protein All-In-One Kit
A$575.00

Key features and details

Quick and efficient isolation of DNA, RNA and protein from the same sample using spin column technology

 

 

SKU: 116544050

Synonyms
SPINeasy DNA/RNA/Protein Extraction Kit; Multianalyte Extraction Kit
Packaging size:
50 Preps
Ships in one Business Day
Product Description

SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica-membrane spin-columns to isolate DNA, RNA and protein components from the same sample, without the use of toxic substances such as phenol and chloroform. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep® Instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. DNA, RNA and proteins are then sequentially purified from the same lysate. Briefly, DNA is adsorbed onto the first spin column, while the flow-through is collected and used for RNA purification. The second spin column captures RNA and the flow-through from this step is used for protein extraction. DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted. In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed and resuspended. Each molecular component is then immediately available for their respective downstream applications.

Kit performance

 

Figure 1 (left): (A) DNA; (B) RNA; (C) protein extracted from each animal tissue using SPINeasy DNA/RNA/Protein All-In-One Kit, analyzed using gel electrophoresis. M: DNA marker; Lane 1: Kidney; Lane 2: Spleen; Lane 3: Liver. 
Figure 2 (right): (A) PCR amplification of DNA and (B) RT-PCR amplification of RNA extracted from various samples using SPINeasy DNA/RNA/Protein All-In-One Kit. M: DNA marker; Lane 1: Kidney; Lane 2: Spleen; Lane 3: Liver; Lane 4: Negative control.

 

 

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Contact us for your SPINeasy DNA/RNA/Protein All-In-One, 5 preps here. Please provide appropriate information so our local representative are able to get back to you soon.

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Application Notes

The SPINeasy DNA/RNA/Protein All-In-One Kit quickly and high-efficiently isolate DNA, RNA and protein from the same sample, without the use of toxic substances.

Usage Statement

Research Use Only (RUO).

Key Applications

PCR, RT-PCR, cDNA synthesis, NGS, Western blots, ELISA , Genomics, Molecular Biology

Specifications
SKU 116544050
Alternate Names SPINeasy DNA/RNA/Protein Extraction Kit; Multianalyte Extraction Kit
Application Notes The SPINeasy DNA/RNA/Protein All-In-One Kit quickly and high-efficiently isolate DNA, RNA and protein from the same sample, without the use of toxic substances.
Base Catalog Number 65440
Product Line SPINeasy
Components 50x Lysing Matrix A, 60 mL Lysis Buffer R, 30 mL Wash Buffer D, 12 mL Wash Buffer R, 10 mL Elution Buffer GD, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 80 mL Protein Precipitant, 100x Column A with collection tube
Hazard Statements Hazard Class 8
Pack Size 50 Preps
Personal Protective Equipment Safety Goggles/Faceshield; Gloves
Sample Type Blood, Cell Culture, Animal Tissue, Swabs
Storage Store at Room Temperature (15-25 °C) in a dry environment
Usage Statement Research Use Only (RUO).
Frequently Asked Questions
Documents

Certificate of Analysis (COA)

Datasheet

Safety Data Sheets

Manuals

Brochures

Protocols