SPINeasy® DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica-membrane spin-column technology, the SPINeasy® DNA Kit for Microbiome can be used for bacterial, body fluids and environmental samples such as soil and stool. With the use of specially formulated Buffer MB1 and Lysing Matrix E in combination with FastPrep® Instruments from MP Biomedicals, an efficient lysis of various samples can be achieved within seconds. Provided in the kit, the Column MB and kit buffers are designed to deliver gDNA of high yield and purity, and compatible with downstream applications such as qPCR, restriction digestion, and sequencing.
Performance
SPINeasy® DNA Kit for Microbiome has been rigorously validated on various types of samples.
Microbial culture samples
The bacterial DNA extracted with SPINeasy® DNA Kit for Microbiome has higher yield as compared to that of competitor kits.
Bronchoalveolar lavage fluid (BALF)
The DNA extracted with SPINeasy® DNA Kit for Microbiome has high yield and good DNA integrity.
Milk
Different types of milk contain different amounts of protein and fat. SPINeasy® DNA Kit for Microbiome has an effective impurity removal technology and is able to extract gDNA from the three types of milk tested.The DNA extracted with SPINeasy® DNA Kit for Microbiome showed high yield and better purity (especially for fresh milk) than that of competitor kits.
Human whole blood
The DNA extracted with SPINeasy® DNA Kit for Microbiome showed high yield and purity.
Inhibitor-Free DNA Ready for Downstream Applications
The DNA extracted with SPINeasy® DNA Kit for Microbiome could be directly used for downstream applications (such as qPCR), suggesting the effectiveness of the kit in removing any inhibitor.
Application Notes
Efficient and unbiased lysis of microbes, including gram positive/negative bacteria, fungi, protozoans, and viruses found in swab, sputum, body fluid, stool, soil, and water samples.
SPINeasy® DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica membrane spin column technology, the SPINeasy DNA Kit for Microbiome can be used for bacterial, body fluids, and environmental samples such as soil and stool.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Genomic, Molecular Biology, PCR, qPCR, Microbiome analysis, NGS, Shotgun sequencing
| SKU | 116553050 |
| Alternate Names | SPINeasy DNA Extraction Kit for Microbiome, gDNA Microbial Extraction Kit |
| Application Notes |
Efficient and unbiased lysis of microbes, including gram positive/negative bacteria, fungi, protozoans, and viruses found in swab, sputum, body fluid, stool, soil, and water samples. SPINeasy® DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica membrane spin column technology, the SPINeasy DNA Kit for Microbiome can be used for bacterial, body fluids, and environmental samples such as soil and stool. |
| Base Catalog Number | 6553050 |
| Product Line | SPINeasy |
| Components | Lysing Matrix E (50 ea), RNase A Solution (2 mL), Buffer MB1 (45 mL), Buffer MB2 (17 mL), Buffer MB3 (21 mL × 2), Buffer MB4 (28 mL), Buffer MB5 (20 mL × 2), Buffer MB6 (10 mL), Column MB (50 ea), 1.5 mL Collection Tubes (50 ea) |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Swabs, sputum, body fluids, stool, and environmental samples (e.g. soil and water) |
| Storage | Store at Room Temperature (15-25℃) in a dry environment |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
