MagBeads FastDNA™ Kit for Feces
SKU: 1165704-CF
The MagBeads FastDNA Kit for Feces allows quick and efficient isolation of high-quality genomic DNA from fresh or frozen human and animal feces in less than 60 minutes.
Samples are placed into Lysing Matrix E tubes and used with FastPrep® Instruments from MP Biomedicals to effectively lyse host cells as well as bacteria, fungi, viruses, protists, and other cells present in fecal samples within 40 seconds.
The kit is also compatible with most of the automated nucleic acid extraction instruments on the market or it can be operated manually. It consists of specially formulated reagents to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from feces and thus allow extraction of highly pure genomic DNA ready for PCR, restriction digestion, electrophoresis, and other desired applications.
Figure 1: gDNA extracted from different feces sample using MagBeads FastDNA® Kit for Feces.
Figure 2: PCR of gDNA extracted from different feces sample using MagBeads FastDNA® Kit for Feces.
M: 1kb plus DNA ladder; Lane 1: swine feces; Lane 2: mouse feces; Lane 3: human feces; Lane 4: chicken feces; Lane 5: bovine feces; Lane 6: elephant feces; Lane 7: negative control.
| SKU | 1165704-CF |
| Product Line | MagBeads |
| Components | 50 x 2 mL Lysing Matrix E, 60 mL pre-wash buffer, 60 mL lysis buffer F1, 60 mL lysis buffer F2, 1 mL Rnase A solution, 15 mL PPS (protein precipitate solution), 18 mL Binding Buffer F, 550 uL magnetic beads, 15 mL wash buffer F1, 30 mL wash buffer F2, 10 mL TE buffer, 100 x 2.0 mL microcentrifuge tubes and 50 x 1.5 mL microcentrifuge tubes. |
| Format | Kit |
| Sample Type | Feces, Fresh or frozen human and animal stool |
| Storage | Magnetic beads should be stored at 2-8°C, the remaining kit contents should be stored at 15-30°C. |
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