DNA extraction of underground water using SPINeasy DNA Kit for water (Cat. No. 116532050)
Hidden water to be purified for DNA extraction
DNA extraction from any water samples would require initial step of filtration or precipitation to collect the microbials. Filtration method requires passage of water samples through a filter paper or membrane to trap the DNA whereas the precipitation method uses ethanol to precipitate nucleic acids in the water sample.
For this experiment, MP Biomedicals decided to put underground water to test with our SPINeasy DNA Kit for Water (Cat. No. 116532050) using filtration method and 5ml tubes.
Overview
Sample Name:
Underground Water
Sample Type:
Water
Challenge :
Samples contain low microbial content, and the sample volume is large.
Featured Materials:
SPINEasy™ DNA Kit for Water, 50 preps
Quick Isolation of genomic DNA from water using silica membrane spin column technology
Methods
Sample was prepared in order of protocol in accordance to product guidelines found here.
Outcome
Trial results data
Electrophoresis chart
Sample 48,49 using SPINeasy DNA Kit for Water（cat #116532050）
Sample 50~59 using FastDNA Spin Kit For Soil（cat #116560200）
Trial Experience:
DNA extraction results was good.
The filter membrane is effective in collecting the microbials and 5ml tubes from SPINeasy DNA Kit for Water helps to get better lysing result.
Conclusion
Key takeaways:
SPINeasy DNA Kit for Water provided better and effective DNA extraction results with the use of filter membrane for effective collection of microbials and the use of 5ml tubes for better lysing results.
Looking To Try Our Extraction Kits?MP Biomedicals is now offering Free Trial Pack Application for the SPINeasy® DNA Kit for Water (5 Preps) to any valid research scientists. |
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