DNA extraction of biological membrane on inner wall of water pipe with SPINeasy DNA Kit for Soil (Cat. No. 116530050)
Caught in a tight situation with low DNA purity from samples in the inner wall of water pipes
Soil genomic DNA extraction kit (Spin Column membrane method) or SPINeasy DNA Kit for Soil is not limited to use for DNA extraction from soil samples. The lysis buffer, elution buffer and wash buffer in the kit can effectively remove any inhibitors that will affect the DNA extraction in other sample types to improve both yield and purity of DNA extraction to facilitate study of microbial diversity and use in downstream application.
Constraint by limited purity of DNA from biofilm on the inner wall of water pipe, the DNA extraction from silt and various microorganisms in the pipe wasn't performing as desired.
Going beyond soil sample types, MP Biomedicals approached the researcher and offered SPINeasy DNA Kit for Soil (Cat. No. 116530050) as an option to improve quality and quantity of theDNA extraction from the samples in the water pipes
Overview
Sample Name:
Biological membrane or biofilm on water pipe inner wall, organic and microbia content in silt and other microorganisms found in the pipe.
Sample Type:
Featured Materials:
SPINeasy™ DNA Kit for Water, 50 preps
Quick Isolation of genomic DNA from water using silica membrane spin column technology
Methods
Sample was prepared in order of protocol in accordance to product guidelines found here.
Outcome
Trial results data
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Conclusion
Key takeaways:
The absorbance ratio A260/230 reached 1.73 suggesting that the DNA purity wasimproved significantly and the customer was satisfied with the results.
Looking To Try Our Extraction Kits?MP Biomedicals is now offering Free Trial Pack Application for the SPINeasy® DNA Kit for Water (5 Preps) to any valid research scientists. |
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