LymphoSep™, lymphocyte separation medium

LymphoSep™, lymphocyte separation medium

SKU 0916922-CF
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A$58.55
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Description

Product Description

Lymphosep - Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface.

  • Maximum yield of mononuclear cells
  • Easy one-step centrifugation
  • >96% cell viability of lymphocytes
  • Low endotoxins
  • Autoclave sterilization

Check out our latest article in Genetic Engineering & Biotechnology News about High-Performance Density Gradient Media for Purification of Leukocytes from Blood.

Application Notes

LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets.

Key Applications

In vitro isolation of lymphocytes from diluted whole blood.

Specifications
SKU 0916922-CF
Application Notes LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets.
Base Catalog Number 1692249,1692254
Format solution
Formulation Details Liquid - Lymphosep is supplied as a ready made sterile aqueous solution of sodium metrizoate (9.6% w/v) and Ficoll® (5.6% w/v).
pH 6.0 - 9.0
Physical Appearance Clear colorless liquid
Presentation Lymphosep® is supplied as a ready made sterile aqueous solution of sodium metrizoate (9.6% w/v) and Ficoll® (5.6% w/v). | Liquid
Product Overview Ready to use quick, simple and reliable medium for the isolation of lymphocytes from whole blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep® interface.
Sterility Sterile
Storage and Handling Store at Room temperature (15-30°C); protect from light