Yeast cell walls are resilient, hence it can become a challenge when trying to extract the intact purified DNA. Many times, the extracted DNA can be damaged or contaminated due to unintuitive workflow. SPINeasy® DNA Kit for Yeast is a high-performance genomic DNA (gDNA) extraction kit which is developed based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from yeast cells, typically in less than 30 minutes. Provided in the kit, Column S and kit buffers are designed to deliver gDNA extracts of high yield and purity; the extracted gDNA is compatible with downstream applications such as PCR amplification, restriction enzyme digestion, and sequencing.
Performance
Figure 1. SPINeasy® DNA Kit for Yeast generates DNA yield that is proportionate to the input sample amount, Saccharomyces cerevisiae (1 x 107 to 4 x 108 cells). 3 μL of purified genomic DNA from a 100 μL eluate were analyzed by electrophoresis on a 1% agarose gel.
Figure 2.
The SPINeasy® DNA Kit for Yeast was used to extract genomic DNA (gDNA) from Saccharomyces cerevisiae. The yield and purity were assessed and compared with three different competitor kits (Q, T, Z). The MP Kit exhibited a purity range exceeding 1.8 while maintaining a high yield. Competitors Q and T yielded high quantities of gDNA but with a lower A260/A230 ratio. Competitor Z yielded both a lower quantity of gDNA and a lower purity ratio. Gel electrophoresis of the extracted gDNA displays maximum band integrity for the MP Kit.
Application Notes
The SPINeasy® DNA Kit for Yeast was designed for rapidly isolating high-quality DNA from yeast samples.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
PCR amplification, restriction enzyme digestion, and sequencing
| SKU | 116557050 |
| Application Notes | The SPINeasy® DNA Kit for Yeast was designed for rapidly isolating high-quality DNA from yeast samples. |
| Base Catalog Number | 6557050 |
| Brand | SPINeasy |
| Components | Lysing Matrix YG (50 x 2.0 mL), Lysis Buffer Y (30 mL), Binding Buffer Y (30 mL), Wash Buffer Y1 (32 mL), Wash Buffer Y2 (4.8 mL), Elution Buffer EB (12 mL), RNase A (280 μL), Column S (50 ea), Collection Tube (50 ea), Elution Tube (50 ea) |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
