Affinity Purified Goat Anti-Rat IgG (H&L), 2 mg
SKU: 0855740
Goat affinity purified antibody to rat IgG (whole molecule) is goat affinity purified antibody to rat IgG (whole molecule) and buffer salts.
Application Notes
Goat affinity purified antibody to rat IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry | ELISA
| SKU | 0855740 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Goat affinity purified antibody to rat IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55740 |
| Concentration | Total Protein: 1.02 mg/mL Active Antibody: 1.0 mg/mL |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Purified |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mg |
| Preparation Method | The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | Rat |
| Specificity | It shows reactivity to rat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | SUGGESTED WORKING DILUTIONS 1:50 - 1:200 for Tissue Staining 1:100 - 1:500 for Immunoblotting 1:400 - 1:2,000 for Elisa |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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