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TBE buffer

TBE buffer

€52.80
Tris-Borate-EDTA electrophoresis buffer, ready-to-use

SKU: 04816721-CF

Synonyms
TBE buffer
CAS Number:
Not applicable
MDL Number:
MFCD00236358
Product Description

Tris-Borate-Edta Buffer

Application Notes

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis. When diluted to a 1X concentration, it yields a TBE buffer solution that contains 100 mM Tris, 90 mM boric acid and 1mM EDTA. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Buffer for DNA and RNA polyacrylamide gel electrophoresis

Specifications
SKU 04816721-CF
Alternate Names TBE buffer
Application Notes TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis. When diluted to a 1X concentration, it yields a TBE buffer solution that contains 100 mM Tris, 90 mM boric acid and 1mM EDTA. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Base Catalog Number 816721
CAS # Not applicable
Format Solution
Hazard Statements H360FD
Personal Protective Equipment Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
Safety Symbol GHS08
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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