Ribonuclease A

Product Description

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.

Application Notes

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples

Specifications

SKU	02193980-CF
Alternate Names	Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
Application Notes	Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Base Catalog Number	193980
Biochemical Physiological Actions	Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
CAS #	9001-99-4
EC Number	232-646-6
Extinction Coefficient	E1%280 = ~7.3
Format	Lyophilized powder, Salt-free and protease-free
Grade	Molecular Biology Grade
Hazard Statements	H317
Isoelectric Point	9.45
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
pH	Optimum pH: 7.0 - 7.5
Preparation Method	Chromatographically Purified
RTECS Number	RF0760000
Solubility	Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Source	Bovine Pancrease
Specific Activity	≥70 Kunitz units/mg
Specificity	The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Unit Definition	One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.