Polysorbate 20
Application Notes
Polysorbate 20 is used as emulsifying agent for the preparation of stable oil-inwater emulsions in pharmaceuticals, cosmetics, cleaning compounds. Polysorbate 20 has been used in preextraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). It has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. It can be used for lysing mammalian cells at a concentration of 0.05 to 0.5%. It is a nonionic detergent widely used in biochemical applications.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Non-ionic detergent
| SKU | 04806576 |
| Alternate Names | Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurate; PEG(20)sorbitan monolaurate; TWEEN® 20 |
| Application Notes | Polysorbate 20 is used as emulsifying agent for the preparation of stable oil-inwater emulsions in pharmaceuticals, cosmetics, cleaning compounds. Polysorbate 20 has been used in preextraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). It has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. It can be used for lysing mammalian cells at a concentration of 0.05 to 0.5%. It is a nonionic detergent widely used in biochemical applications. |
| Base Catalog Number | 806576 |
| CAS # | 9005-64-5 |
| Critical Micelle Concentration | 0.06 mM (60 mg/L or 6 × 10-3 mole/L)(Lit.) |
| Format | Liquid |
| Molecular Weight | 522.676 g/mol |
| Pack Size | 100 g |
| Personal Protective Equipment | Eyeshields, Gloves |
| Refractive Index | n20/D 1.468(Lit.) |
| RTECS Number | TR7400000 |
| Solubility | Soluble in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>, <a class="pubchem-internal-link CID-8857" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethyl%20acetate">ethyl acetate</a> and <a class="pubchem-internal-link CID-31275" href="https://pubchem.ncbi.nlm.nih.gov/compound/dioxane">dioxane</a>. Insoluble in mineral oil and petroleum ether |
| Specific Gravity | 1.08 - 1.13 |
| Sterilization of Solutions | Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Polysorbate 20 may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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