MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-32)

The MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-32™) is a high-performance RNA extraction kit designed for formaldehyde- or paraformaldehyde-fixed, paraffin-embedded (FFPE) tissues, optimized for use with the MPure-32™ aNAP System or similar automated nucleic acid purification platforms. This kit is based on magnetic-bead technology; it enables convenient isolation of RNA from a variety of FFPE tissues. 
RNA obtained from multiple types of tissue using this kit has shown high integrity and purity and is immediately ready for use in downstream applications, including real-time PCR and next-generation sequencing (NGS). 
The kit incorporates specialized formulations that address the unique challenges of FFPE samples. During formalin fixation, nucleic acids undergo crosslinking with proteins and chemical modifications that lead to fragmentation. The MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-32™) is optimized to reverse formaldehyde-induced crosslinking.
Due to fixation and embedding procedures, nucleic acids in FFPE samples are often heavily fragmented and modified by formaldehyde. While the MagBeads FastRNA Kit for FFPE is optimized to minimize the effects of the formaldehyde modification, RNA purified with this kit is not recommended for downstream applications that require full-length RNA.

Performance

MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-32™) consistently delivered higher yield than all competitors’ kits across most sample types. It also demonstrated better RNA purity compared to all competitors’ kits. 

 

 
 
 Figure 1: Quality and quantity of RNA extracted from various FFPE tissues using the MagBeads FastRNA Kit for FFPE and three competitor kits. RNA yield, A260/A230 ratio, and A260/A280 ratio were determined by spectrophotometry using RNA extracted from 2 mg of each tissue sample.

RNA extracted using this kit is compatible with real-time qPCR assays. Across five different tissue RNA samples tested, RNA isolated with the MagBeads FastRNA Kit for FFPE consistently yielded the lowest Ct values.

Figure 2: Amplifiability of RNA extracted with MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-32™). qPCR threshold cycle (Ct) values were compared using equal input amounts of RNA (30 ng). Targets were amplified using SYBR Green technology.

Application Notes

The MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-32™) is intended for purification of RNA from FFPE samples.

Usage Statement

Research Use Only (RUO)

Key Applications

PCR, real time PCR, and sequencing