MagBeads FastRNA Kit for Blood
SKU: 1165860-CF
MagBeads FastRNA® Kit for Blood is a scalable and automatable (automatable versions available cat. no.117039100 & cat. no.117039200) magnetic bead-based RNA purification technology that is designed for total isolation of RNA from whole blood preserved in different anticoagulants (EDTA, Heparin and sodium citrate). RNA degradation prevention is the backbone of RNA purification. The use of our specially formulated Buffer MBL containing huge amounts of chaotropic ions omits the need of excessive homogenization and enables highly efficient lysis with the immediate inactivation of RNases, which are typically present in most biological materials. The MagBeads FastRNA® Kit for Blood simplifies the RNA purification process by eliminating the need for complicated erythrocyte lysis and isolation of leukocyte pellet, making it hassle-free and time efficient. Contaminated DNA is removed by a DNase I treatment step. Simplified dual washing steps largely remove salts, metabolites, and macromolecular cellular components. Pure RNA extracts of high yield and purity, compatible with downstream applications such as RT-qPCR amplification, are then eluted under low ionic strength conditions with RNase-free water.
Figure 1: High Quality RNA Purification. MagBeads FastRNA® Kit for Blood delivers high yield and purity with consistent RNA extraction efficiency.
Figure 2: Evaluation of the performance of the MagBeads FastRNA® kit for blood compared to Competitor M and Competitor MN . RNA was independently extracted (three extractions of quadruplicates each) from MagBeads FastRNA® kit for Blood (MP), Competitor M or Competitor MN. The RNA yield, purity (260/230 ratio) integrity, and RIN were assessed using spectrophotometry and Agilent Technologies. In addition, the extracted RNA was also assessed for its amplifiability using qPCR and the Ct values given by MP are earlier than Competitor M. The horizontal bars indicate the median value.
Application Notes
The MagBeads FastRNA Kit for Blood is designed for magnetic bead-based purification of RNA from blood samples.
Usage Statement
Research Use Only (RUO)
Key Applications
PCR, qPCR amplification
| SKU | 1165860-CF |
| Application Notes | The MagBeads FastRNA Kit for Blood is designed for magnetic bead-based purification of RNA from blood samples. |
| Base Catalog Number | 1165860 |
| Product Line | MagBeads |
| Components | DNase I (11 μL), DNase I Buffer (700 μL), Proteinase K (110 μL), Magnetic Beads M (110 μL), Buffer MBL (1.1 mL), Buffer MBB (500 μL), Buffer MW1 (1.5 mL), Buffer MW2 (590 μL), RNase-free Water (550 μL) |
| Format | Kit |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Blood, Whole Blood |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Research Use Only (RUO) |
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