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Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
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Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples
|Alternate Names||Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase|
|Application Notes||Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.|
|Base Catalog Number||193980|
|Biochemical Physiological Actions||Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.|
|Extinction Coefficient||E1%280 = ~7.3|
|Format||Lyophilized powder, Salt-free and protease-free|
|Grade||Molecular Biology Grade|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|pH||Optimum pH: 7.0 - 7.5|
|Preparation Method||Chromatographically Purified|
|Solubility||Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.|
|Specific Activity||≥70 Kunitz units/mg|
|Specificity||The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.|
|Unit Definition||One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0.|