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4′,6-Diamidino-2-phenylindole dihydrochloride

4′,6-Diamidino-2-phenylindole dihydrochloride
€13.60

Key features and details

4′,6-Diamidino-2-Phenylindole Dihydrochloride

SKU: 02157574-CF

Synonyms
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride
CAS Number:
28718-90-3
Molecular Formula:
C16H15N5·2HCl
Molecular Weight:
350.247 g/mol
Beilstein Registry Number:
4894417
EC Number:
249-186-7
MDL Number:
MFCD00012681
Product Description

4′,6-Diamidino-2-Phenylindole Dihydrochloride

Application Notes

4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Photofootprinting of DNA

Specifications
SKU 02157574-CF
Alternate Names 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride
Application Notes 4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Base Catalog Number 157574
Beilstein Registry Number 4894417
Biochemical Physiological Actions Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
CAS # 28718-90-3
EC Number 249-186-7
Extinction Coefficient (263 nm): 30 (water)(Lit.)
Format Powder
Molecular Formula C16H15N5·2HCl
Molecular Weight 350.247 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Typical Working Concentration For vital staining, 1 ug/mL in cell growth medium was used.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificate of Analysis (COA)