NEW Product: MagBeads FastDNA™ Kit for Feces request your FREE sample
Product Description
4′,6-Diamidino-2-Phenylindole Dihydrochloride
Application Notes
4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Photofootprinting of DNA
Specifications
| SKU | 02157574-CF |
| Alternate Names | 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride |
| Application Notes | 4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma. |
| Base Catalog Number | 157574 |
| Beilstein Registry Number | 4894417 |
| Biochemical Physiological Actions | Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. |
| CAS # | 28718-90-3 |
| EC Number | 249-186-7 |
| Extinction Coefficient | (263 nm): 30 (water)(Lit.) |
| Format | Powder |
| Molecular Formula | C16H15N5·2HCl |
| Molecular Weight | 350.247 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| Typical Working Concentration | For vital staining, 1 ug/mL in cell growth medium was used. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
![MP COA [157574] lot ID: 1010C](https://www.mpbio.com/static/version1656588924/frontend/Mpbio/default/en_US/images/document.png "MP COA [157574] lot ID: 1010C")