Fluorescein-Conjugated Goat IgG Fraction To Mouse Immunoglobulins (IgG, IgA, IgM)

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat IgG Fraction to Mouse Immunoglobulins (IgG, IgA, IgM) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

SKU	0855499
Antibody Type	Polyclonal Antibody
Application Notes	Fluorescein-Conjugated Goat IgG Fraction to Mouse Immunoglobulins (IgG, IgA, IgM) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Base Catalog Number	55499
Concentration	Total Protein: 15.9 mg/mL
Conjugate	FITC
Format	lyophilized
Formulation Details	Lyophilized
Grade	Molecular Grade
Host	Goat
Immunogen	Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.
Isotype	IgG, IgA, IgM
Pack Size	2 mL
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity	Purified
Solubility	Soluble in water
Species Reactivity	Mouse
Specificity	This product shows reactivity to mouse immunoglobulins (IgG; IgA; IgM); cross-reactivity to other species may exist.
Typical Working Concentration	Suggested working dilutions: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immunoblotting; 1:1,000 - 4,000 for ELISA
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.