Anti-human IgA (alpha chain) goat F(Ab')2 fragment, fluorescein-conjugated, 2 mL

Product Description

Fluorescein-conjugated Goat F(ab')₂ fragment to Human IgA is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')₂ fragment to human IgA (α chain) and buffer salts.

Application Notes

Fluorescein-conjugated goat F(ab')₂ fragment to human IgA (α chain) is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Note: Affinity purified antibodies and their F(ab')2 fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. A dilution of 1:100 was used for immunohistochemistry of cryosections of human sural nerves.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications

SKU	0855178
Antibody Type	Polyclonal Antibody
Application Notes	Fluorescein-conjugated goat F(ab')₂ fragment to human IgA (α chain) is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Note: Affinity purified antibodies and their F(ab')2 fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. A dilution of 1:100 was used for immunohistochemistry of cryosections of human sural nerves.
Base Catalog Number	55178
Concentration	Total Protein: 13.8 mg/mL; Antibody Protein: 6 mg/mL
Conjugate	FITC
Format	lyophilized
Formulation Details	Lyophilized
Grade	Molecular Grade
Host	Goat
Immunogen	Purified human IgA
Pack Size	2 mL
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Purity	Purified
Solubility	Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity	Human
Specificity	This product shows reactivity to human IgA; cross-reactivity to other species may exist. Antibody activity to IgG; IgM; or non-immunoglobulin serum proteins is not present.
Typical Working Concentration	Dilution Recommendations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.