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Fluorescein-conjugated Goat IgG Fraction to Human Complement C1q is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human complement C1q and buffer salts.
Fluorescein-conjugated Goat IgG Fraction to Human Complement C1q is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
|Alternate Names||Complement component C1q A chain antibody; Complement component C1q B chain antibody; Complement component C1q C chain antibody;|
|Antibody Type||Polyclonal Antibody|
|Application Notes||Fluorescein-conjugated Goat IgG Fraction to Human Complement C1q is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.|
|Base Catalog Number||55166|
|Immunogen||C1q isolated from the normal human serum.|
|Pack Size||2 mL|
|Preparation Method||Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.|
|Solubility||Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|
|Specificity||This product shows reactivity to human complement C1q; cross-reactivity to other species may exist. Antibody activity to other complement components or serum proteins is not present.|
|Typical Working Concentration||Dilution Reccomondations: 1:100 - 1:400 for Tissue Staining; 1:1,000 - 1:4,000 for ELISA/FIA|