2XYT-broth agar, capsules
YT media formulations are optimized for the growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.
2xYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant Escherichia coli strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
|Alternate Names||Bacteriophage Media|
|Application Notes||2xYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant Escherichia coli strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.|
|Base Catalog Number||3012231|
|Cells Tested||E. coli|
|Certifications||Molecular Biology Certified Bacterial Growth Medium; Gelatin capsules are produced in a facility that meets the FDA’s recent guidance as being derived from BSE-free herds that are born, raised, and slaughtered in the United States.|
|Formulation Details||Contents Per Liter: 16 g Tryptone-B; 10 g Yeast Extract-B; 5 g NaCl; 15 g Agar-B|
|Kit Compatibility||Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.9 ± 0.3 at 25°C.|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in 2xYT-Broth formulations. Chosen reagents are combined in the proper proportions according to the 2xYT-Broth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 46 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).|
|Product Families Description||Bacteriophage manipulation is easier with the right media. MP Biomedicals offers media specifically designed for bacteriophage propagation, maintenance and manipulation. Quality components such as Tryptone-B, Yeast extract, NZ-amine, amino acids and sodium or magnesium salts are added in specialty formulations for Lambda phage, M13 phage and other filamentous ssDNA bacteriophages. MP Bio also offers formulations for competent cell preparation of phage containing lines. Other choices in formulation include with, or without agar, and packaging choices are: |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!|
|Product Overview||2xYT-Broth has the composition of 31 grams per liter Contents/liter: 16 g tryptone, 10 g yeast extract, 5 g NaCl|
|Recommended Replacement For||YT-Broth|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology|
|Solubility||Soluble in water (46 g/L).|
|Storage and Handling||Store dry 2xYT-Broth Powder Medium at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|
|Usage Statement||Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.|
|Grade||Molecular Biology Certified™|