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SuperBroth is enriched medium with high levels of tryptone and yeast extract. Due to the high levels of peptides, amino acids, vitamins and cofactors high yields of E. coli cells and lambda bacteriophages are obtained.
Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of Lambda phage
|Alternate Names||Bacteriophage Media|
|Application Notes||Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.|
|Base Catalog Number||3010031|
|Boiling Point||1461 °C (2662 °F)(Lit.)|
|Components||35 g tryptone, 20 g yeast extract, 5 g NaCl|
|Formulation Details||Contents Per Liter: 35 g Tryptone-B; 20 g Yeast Extract; 5 g NaCI.|
|Grade||Molecular Biology Certified™|
|Media Type||Super Broth|
|Pack Size||1 kg|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.6 ± 0.2 at 25°C.|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in SuperBroth formulations. Chosen reagents are combined in the proper proportions according to the Superbroth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 60 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).|
|Solubility||Soluble in water (60 g/L).|
|Usage Statement||Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.|