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Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes this the method of choice for the high efficiency transformation needed in two hybrid screens.
Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.
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Transformation of yeast cells
|Alternate Names||High Efficiency Yeast Transformation Kit|
|Application Notes||Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.|
|Base Catalog Number||2200200|
|Components||Sterile TE Buffer pH 7.5 |Lithium/Cesium Acetate |Carrier DNA 10 mg/mL Sterile |Sterile Solution Histamine |TE/Cation MIXX |PEG |Sterile Solution SOS|
|Format||All necessary reagents|
|Pack Size||250 Preps|
|Personal Protective Equipment||Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves. Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields. Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Recommended Alternate||EZ-Yeast™ Transformation Kit|
|Safety Symbol||GHS07 |GHS08|
|Sample Type||Overnight yeast culture or colonies from plates|
|Sample Volume||500 µL or 3-4 mm colony|
|Unit Definition||≥ 104 transformants/µg plasmid DNA|