SPINeasy^® RNA Kit for Bacteria, 50 preps

SPINeasy RNA Kit for Bacteria is a silica -membrane spin -column kit that enables quick and convenient purification of total RNA from microbial samples, including bacteria and fungi. Included in the kit is our specially formulated RNASS solution that stabilizes and protects RNA. The use of Lysis Buffer R and Lysing Matrix B in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of microbial samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Total RNA of high quality and integrity can be purified typically within 40 minutes and is immediately available for RT-PCR and other downstream applications.

Performance Data

SPINeasy RNA Kit for Bacteria has been rigorously tested for its performance using a variety of samples. The following are a few examples of the results of RNA extraction

M: DNA marker Lane 1: E. coli Lane 2: S. aureus

Figure 1: Agarose gel electrophoresis demonstrates integrity of total RNA

M: DNA marker Lane 1: E. coli Lane 2: S. aureus Lane 3: Negative control

Figure 2: RT-PCR performance of bacterial RNA samples extracted using SPINeasy RNA Kit for Bacteria.

Application Notes

The SPINeasy RNA Kit for Bacteria quickly and efficiently isolates high-quality, total RNA from G+ and G- bacteria using a specialized Lysing Matrix B for cell lysis and SPIN columns for the purification process.

Usage Statement

Research Use Only (RUO).

Key Applications

Total RNA extracted from SPINeasy RNA Kit for Bacteria can be used in several downstream applications, including RT-PCR, qPCR, Sequencing (NGS), Microbial transcriptomics, RNA-Seq, Northern Blot Analysis, Genomics, Molecular Biology