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Top Lysing Matrix Accessories

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Brochure

Sample Preparation Brochure

Find a world of dependable sample preparation products for generating high-quality DNA, RNA, and proteins—FastPrep systems, lysing matrix tubes, kits, and more.

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Brochure

Environmental Brochure

From sample preparation to molecular analysis, find end-to-end solutions for working with environmental samples.

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Brochure

Challenges in Plant Research

Discover our range of reagents and instruments designed to overcome the many challenges of plant research.

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Brochure

Nucleic Acid Extraction

Explore how MPBio can assist you in isolating DNA and RNA from your samples.

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The ABCs of Lysing Matrices

With a wide range of bead sizes, shapes, and compositions, MP Bio offers a dependable lysing matrix for every application.

Size

The smaller the particles used in the grinding media, the smaller the average particle size and the smaller the lowest-limiting particle size produced during pulverization. Matrix particle size should be selected based upon the size of the particles you wish to obtain in your lysate.

Shape

The shape of the grinding media is a major determining factor in how cells are disrupted. Dull media, such as spherical beads, utilize cascade impaction (hammering) as the main force for cell lysis. Sharp and angular shaped media will primarily generate mechanical shear forces (chopping and cutting) which can quickly open difficult cell walls, grind fibrous or elastic animal tissue, or crack spores or oocytes. Shear forces are preferable when isolating stable molecules such as DNA, stable proteins, structural polysaccharides and small molecules or metabolites. RNA and certain easily denatured proteins can be quickly degraded by shear forces, so care needs to be taken when using angular media. For isolation of these molecules, smooth impactor grinding media can be much more forgiving.

Hardness, Density, and Composition

The composition determines two very important qualities: hardness and density, both of which are inherent physical properties derived from the molecular composition of the matrix particle. The hardness must be greater than that of the sample being pulverized, with higher hardness values being more effective at disrupting hard and brittle cell membranes. Hardness and density values help optimize lysis efficiency while preserving the integrity of the analytes of interest.

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Lysing Matrix
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