SPINeasy DNA Kit for Blood is a high-performance genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from whole blood preserved in different anticoagulants (EDTA, heparin, and sodium citrate), typically in less than 30 minutes. It also allows easy gDNA isolation from coagulated blood when using the FastPrep-24 5G homogenizer with lysing matrix A or D tubes (recommended for soft tissues, and not included with this kit). The use of our specially formulated Lysis Buffer BL enables highly efficient lysis of various liquid samples including plasma, serum, saliva, and cell culture medium. Column S1 and kit buffers (provided in the kit) are designed to deliver gDNA extracts of high yield and purity, compatible with downstream molecular applications such as PCR and NGS.
Performance Data
SPINeasy DNA Kit for Blood has been validated for its performance using various biological fluids. The following data demonstrate the gDNA yields obtained from various sample sources. Results demonstrate that gDNA extracted from all sample sources are of high yield and purity (Table 1, Figure 1)
Table 1: Quality and quantity of gDNA extracted from various sample types using SPINeasy DNA Kit for Blood.
*Sample processed using homogenization method via Fastprep-24 5G with lysing matrix.
**Sample preserved in preservation buffer (SPS, provided in SPINeasy DNA Kit for Saliva)
Figure 1. gDNA extracted from various samples using SPINeasy DNA Kit for Blood. The purified DNA was analyzed on a 1% agarose gel by electrophoresis. M: DNA marker, DL15000; 1-2: EDTA; 3-4: Heparin; 5-6: Sodium Citrate; 7-8: Blood Clot; 9-10: Saliva; 11-12: Cell Culture Media
Application Notes
For the extraction of high quality gDNA from a variety of sample sources, including fresh, frozen, and anticoagulated blood; plasma; serum; and cell culture medium for downstream applications such as restriction digestion, PCR and NGS analysis.
Usage Statement
Research Use Only (RUO)
Key Applications
Genomics; Molecular Biology; PCR, qPCR, Microbiome analysis, NGS; shotgun sequencing
| SKU | 116552050 |
| Alternate Names | Spin-column DNA Extraction Kit from Blood, gDNA Extraction kit for blood |
| Application Notes | For the extraction of high quality gDNA from a variety of sample sources, including fresh, frozen, and anticoagulated blood; plasma; serum; and cell culture medium for downstream applications such as restriction digestion, PCR and NGS analysis. |
| Base Catalog Number | 52050 |
| Product Line | SPINeasy |
| Components | Lysis Buffer BL, Wash Buffer BW1, Wash Buffer BW2, Elution Buffer EB, Column S, 2.0 mL collection tubes,1.5 mL microcentrifuge tubes, Proteinase K, Proteinase K Buffer |
| Format | Kit |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Gloves |
| Sample Type | Blood, Saliva, Cultured Cells |
| Sample Volume | 200 μL of Human blood, serum, plasma, saliva, or cell culture media |
| Storage | 15-25 ℃ |
| Usage Statement | Research Use Only (RUO) |
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