MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96)
SKU: 117034500
The MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™) is a high-performance RNA extraction kit designed for formaldehyde- or paraformaldehyde-fixed, paraffin-embedded (FFPE) tissues and optimized for use with the MPure-96™ aNAP System. Based on magnetic bead technology, the kit enables convenient and efficient RNA isolation from a wide range of FFPE tissue types.
RNA obtained from multiple types of tissue using this Kit has shown high integrity and purity and is immediately ready for use in downstream applications, including real-time PCR and next-generation sequencing (NGS).
The kit incorporates specialized formulations that address the unique challenges of FFPE samples. During formalin fixation, nucleic acids undergo crosslinking with proteins and chemical modifications that lead to fragmentation. The MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™) is optimized to reverse formaldehyde-induced crosslinking.
Due to fixation and embedding procedures, nucleic acids in FFPE samples are often heavily fragmented and modified by formaldehyde. While the MagBeads FastRNA Kit for FFPE is optimized to minimize the effects of the formaldehyde modification, RNA purified with this kit is not recommended for downstream applications that require full-length RNA.
Performance
MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™) has been rigorously tested for its performance. RNA was extracted from a variety of FFPE tissues (2 mg) using the MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™). The resulting RNA demonstrated exceptional purity, with A260/A280 values ranging from 2.0 to 2.2 and A260/A230 ratios exceeding 1.7.
Figure 1: Quality and quantity of RNA extracted from various FFPE tissues using the MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™). RNA yield, A260/A230 ratio, and A260/A280 ratio were determined by spectrophotometer using RNA extracted from 2 mg of each tissue.
Figure 2: Amplifiability of RNA extracted with MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™). qPCR threshold cycle (Ct) values were measured using equal input amounts of RNA (30 ng). Targets were amplified using SYBR Green technology.
Application Notes
The MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™) is intended for purification of RNA from FFPE samples.
Usage Statement
Research Use Only (RUO)
Key Applications
PCR, real-time PCR, and sequencing
| SKU | 117034500 |
| Application Notes | The MagBeads FastRNA Kit for FFPE (Ready-to-Use for MPure-96™) is intended for purification of RNA from FFPE samples. |
| Base Catalog Number | 7034500 |
| Product Line | MPure-96 |
| Components | Sample Plate, Wash Plate 1, Wash Plate 2, Wash Plate 3, Elution Plate, DNase Plate, Proteinase K, Protease Dissolve Buffer, DNase I, Buffer DPS, Buffer FRL, Buffer MW1, 96 Spin Tips |
| Pack Size | 96 Preps |
| Personal Protective Equipment | Safety Goggles/Face shield; Gloves |
| Sample Type | FFPE |
| Storage | DNase I, Proteinase K, and Magbeads Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temp |
| Usage Statement | Research Use Only (RUO) |
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