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GNOME® DNA Isolation kit
The GNOME® DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells. Each preparation with the GNOME® kit yields up to 100 µg of genomic DNA. The DNA isolated by the GNOME¨ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.
The GNOME® DNA Isolation Kit is a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types. No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.
Isolation of high molecular weight DNA from a variety of sources
|Alternate Names||DNA Purification|
|Application Notes||The GNOME® DNA Isolation Kit is a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types. No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.|
|Base Catalog Number||2010400|
|Components||Cell Lysis/Denaturing solution | RNase Mixx | Protease Mixx | Salt out Mixture | Cell Suspension solution.|
|Format||All necessary reagents|
|Personal Protective Equipment||Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Cell Lysis/Denaturing Solution: pH range= 6.2-6.9 |Salt-out Mixture: pH range= 6.0-6.8 |Cell Suspension Solution: pH range= 8.0-8.2|
|Preparation Method||Sample preparation protocols provided in kit insert. The yield of genomic DNA and the speed of the protocol are greatly enhanced through the use of the FastPrep®System.|
|Safety Symbol||GHS05 |GHS07 |GHS08|
|Sample Type||Blood, Tissue, Yeast, Bacteria, Plant|
|Sample Volume||20-50 mg wet weight bacterial cell pellet | up to 250 mg plant tissue |2-5 x 107 nucleated white blood cells |50-250 mg mammalian tissue |~1-5 x 108 yeast cell pellet|