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MERmaid® Spin Kit

MERmaid® Spin Kit

Synonyms Purification of small molecular weight DNA

SKU 111105200-CF
$122.00
In Stock
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Description

Product Description

MERmaid® SPIN Kit is designed for rapid purification of small MW single-stranded or double-stranded DNA (10 bp to 200 bp) from agarose or polyacrylamide gels, nucleotides, salts, proteins, or other undesirable components of a mixture. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Application Notes

The MERmaid SPIN Kit is designed for purifying small MW DNA away from gel material, nucleotides, salts, proteins, or other undesirable components of a mixture. Single-stranded or double-stranded DNA oligomers or PCR products of 10-200 nucleotides in length are readily purified from agarose and polyacrylamide gels or from solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Rapid purification of DNA

Specifications
SKU 111105200-CF
Alternate Names Purification of small molecular weight DNA
Application Notes The MERmaid SPIN Kit is designed for purifying small MW DNA away from gel material, nucleotides, salts, proteins, or other undesirable components of a mixture. Single-stranded or double-stranded DNA oligomers or PCR products of 10-200 nucleotides in length are readily purified from agarose and polyacrylamide gels or from solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Base Catalog Number 1105200
Brand MERmaid™
Components GLASSFOG™ SPIN Bind Solution |MERmaid™ SPIN High Salt Wash |MERmaid™ SPIN Ethanol Wash Concentrate |MERmaid™Elution Solution |50x Electrophoresis Buffer |BioGel™ |MERmaid™ SPIN Filters |Elution Catch Tubes |Label Block.
Format GLASSFOG™ slurry plus SPIN filter
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Preparation Method Before first use, add the 22.5 mL of 200 proof (100% ethanol) to the MERmaid™ SPIN Ethanol Wash Concentrate (2.5 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C.
Safety Symbol GHS07
Sample Source Gels and Solutions
Sample Type Enzymatic Reactions, Solutions, Agarose Gels, Polyacrylamide Gels
Sample Volume Up to ~300 mg gel slice or up to ~300 µl DNA solution
Unit Definition Each prep will bind up to 10 μg of either ssDNA or dsDNA.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.