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GENECLEAN® III Kit, 600 Preps

GENECLEAN® III Kit, 600 Preps

Synonyms DNA Clean-Up Kit

Packaging size: 600 Preps

SKU 111001600
$366.00
In Stock
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Description

Product Description

The GENECLEAN® III kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications.

Application Notes

The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA

Specifications
SKU 111001600
Alternate Names DNA Clean-Up Kit
Application Notes The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.
Base Catalog Number 1001600
Brand GENECLEAN®
Components NaI| New wash concentrate| TBE modifier| EZ-Glassmilk™| Label Block| Elution Solution| BioFloat™ floating incubation rack
Format Geneclean® slurry
Pack Size 600 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Above 500 bp: 4.5 M NaI pH 7.0 - 7.4| Between 200 - 500 bp: 4.5 M NaI pH 6.0| NEW Wash: pH Range: 7.4 - 8.0| TBE Modifier: pH 5.8 ± 0.2
Preparation Method Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension.
Recommended Alternate GENECLEAN® SPIN Kit
Safety Symbol GHS05, GHS06
Sample Volume Up to ~0.5 ml gel slice by volume or ~1.0 ml solution
Unit Definition One µL of EZ-GLASSMILK™ will bind with one µg of DNA in 4.5 M Nal in 5 minutes at room temperature.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.