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IgG1 (Kappa) MOPC 21, mouse myeloma, purified, 1 mg

IgG1 (Kappa) MOPC 21, mouse myeloma, purified, 1 mg

Synonyms Mouse IgG1/k

MDL Number: MFCD00164798

Packaging size: 1 mg

SKU 0850327
$200.15
In Stock
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Description

Product Description

Product is purified mouse IgG1 (K) and buffer. Mouse IgG1 (K) is purified from clarified mouse MOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | ELISA | Control

Specifications
SKU 0850327
Alternate Names Mouse IgG1/k
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Base Catalog Number 50327
Clone Name MOPC 21
Concentration Total Protein: 1.2 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Mouse
Isotype IgG
Pack Size 1 mg
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method Mouse IgG1 (K) is purified from clarified mouse MOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
Purity Affinity Purified
Solubility Soluble in Water
Specificity The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG1. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG1; no trace of IgG2a; IgG2b; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Sterility Filtered through a .22 µm filter
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.