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IgG1 (Kappa), Clone Mopc 21, mouse ascites, 5 mg

IgG1 (Kappa), Clone Mopc 21, mouse ascites, 5 mg

Packaging size: 5 mg

SKU 0850341
$173.25
In Stock
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Description

Product Description

[MOPC 21]
Ascites fluid, collected from Balb/c mice which carry plasmacytomas subcutaneously, are clarified by centrifugation and filtration. It is dialyzed, adjusted to yield at least 5 mg of immunoglobulin, then vialed and lyophilized. Clarified ascites are tested for immunoglobulin by immunoelectrophoresis using antisera specific for the particular type and isotype. Approximately 10% of the total protein is immunoglobulin (including trace levels of the host's immunoglobulins), 80% is albumin, and 10% is complement and other proteins.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays

Specifications
SKU 0850341
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Base Catalog Number 50341
Clone Name MOPC 21
Conjugate Unconjugated
Formulation Details Lyophilized
Host Mouse
Isotype IgG1
Pack Size 5 mg
Solubility Soluble in Water
Specificity The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Each ascites is tested for immunoglobulin content by radial immunodiffusion with specific antisera following precipitation by ammonium sulfate (See Protocol for mmunoglobulin Precipitation). In addition; the ascites contains the normal threshold of mouse immunoglobulins and ascites proteins
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.