Puromycin Dihydrochloride
Application Notes
Puromycin is Nucleoside antibiotic & protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3′-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells.Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Antibiotic
| SKU | 02100552-CF |
| Alternate Names | 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine |
| Application Notes | Puromycin is Nucleoside antibiotic & protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3′-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells.Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. |
| Base Catalog Number | 100552 |
| Beilstein Registry Number | 3853613 |
| Biochemical Physiological Actions | Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3'-terminal end of the aminoacyl-tRNA.Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days. |
| CAS # | 58-58-2 |
| EC Number | 200-387-8 |
| Extinction Coefficient | 20.3 (275 nm, 0.1 N NaOH), 19.5 (268 nm, 0.1 NHCl) (Lit.) |
| Hazard Statements | H302 |
| Molecular Formula | C22H29N7O5 · 2HCl |
| Molecular Weight | 544.434 g/mol |
| Personal Protective Equipment | Dust mask, Eyeshields, Gloves |
| pKa | 6.8, 7.2 (Lit.) |
| RTECS Number | AU7355000 |
| Safety Symbol | GHS07 |
| Solubility | Soluble (NTP, 1992) |
| Source | Streptomyces albo-niger |
| Typical Working Concentration | The recommended working concentration in eukaryotic cell culture is 1-10 µg/mL. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | 268 ± 2 nm (λ max (0.1N HCl) |
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