O-Nitrophenyl-β-D-galactopyranoside
SKU: 02102473-CF
O-Nitrophenyl-Β-D-Galactopyranoside
Application Notes
o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Substrate for β-galactosidase
| SKU | 02102473-CF |
| Alternate Names | o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG |
| Application Notes | o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity. |
| Base Catalog Number | 102473 |
| Beilstein Registry Number | 92207 |
| Biochemical Physiological Actions | β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period. |
| CAS # | 369-07-3 |
| EC Number | 206-716-1 |
| Format | Crystalline |
| Molecular Formula | C12H15NO8 |
| Optical Rotation | -66 ° ± 7 ° (c=1, H2O) |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | 262 ± 3 nm λ max (H2O) |