Nuc-Off Nucleases Removal Spray

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SKU:  1124604-CF

Key features and details

  • Nuclease๋ฅผ ์ œ๊ฑฐํ•˜๊ธฐ ์œ„ํ•ด ์‚ฌ์šฉํ•˜๋Š” ๋ฌด๋…์„ฑ spray.

  • ํ‘œ๋ฉด์— ๋ฐ”๋กœ ์ ์šฉ์ด ๊ฐ€๋Šฅํ•˜๋ฉฐ, 5๋ถ„๋‚ด ์ œ๊ฑฐ ์™„๋ฃŒ

  • ์ดˆ์ž๋ฅ˜, ํ”Œ๋ผ์Šคํ‹ฑ, ๋ผํ…์Šค, Stainless steel ๋“ฑ ๋‹ค์–‘ํ•œ ํ‘œ๋ฉด์— ์ ์šฉ๊ฐ€๋Šฅ

  • Nucleic acids์˜ ์•ˆ์ •์„ฑ์€ ์œ ์ง€ํ•˜๋ฉด์„œ DNase์™€ RNase๋Š” ํšจ์œจ์ ์œผ๋กœ ์ œ๊ฑฐ

Product Description

MP Biomedicals์˜ Nuc-Off Nucleases Removal Spray๋Š” RNase์™€ DNase๋ฅผ ๋น ๋ฅด๊ณ  ํšจ์œจ์ ์œผ๋กœ ์ œ๊ฑฐํ•˜๊ธฐ ์œ„ํ•œ ready-to-use solution์ž…๋‹ˆ๋‹ค. ์ด ๋ฌด๋…์„ฑ ๋ฐ ๋น„๋ถ€์‹์„ฑ ์Šคํ”„๋ ˆ์ด ํƒ€์ž… ์†”๋ฃจ์…˜์€ ์‹คํ—˜์‹ค ์†Œ๋ชจํ’ˆ, ๊ธฐ๊ธฐ ๋˜๋Š” ๊ธฐํƒ€ ๋„๊ตฌ์˜ ํ‘œ๋ฉด์—์„œ nuclease๋ฅผ ํšจ๊ณผ์ ์œผ๋กœ ์ œ๊ฑฐํ•˜์—ฌ RNA ๋˜๋Š” DNA ๊ด€๋ จ ์‹คํ—˜์„ ์œ„ํ•œ ๊นจ๋—ํ•œ ํ™˜๊ฒฝ์„ ์กฐ์„ฑํ•ฉ๋‹ˆ๋‹ค. ์‹คํ—˜์—์„œ false positive ๋˜๋Š” false negative ๊ฒฐ๊ณผ๋ฅผ ์œ ๋ฐœํ•  ์ˆ˜ ์žˆ๋Š” nuclease ์˜ค์—ผ์„ ๋ง‰๊ธฐ ์œ„ํ•ด์„œ๋Š” ํ”ผํŽซ, ํด๋ฆฐ๋ฒค์น˜ ํ‘œ๋ฉด, ์‹คํ—˜ ์žฅ๋น„๋“ค์„ ์ด spray๋กœ ์ •๊ธฐ์ ์œผ๋กœ ๋‹ฆ์•„์ฃผ๋Š” ๊ฒƒ์ด ํ•„์ˆ˜์ ์ž…๋‹ˆ๋‹ค. 

์ œํ’ˆ ์„ฑ๋Šฅ

 

 

๊ทธ๋ฆผ 1. RNase์™€ DNase ์ œ๊ฑฐ์—์„œ MP Biomedicals Nucleases Removal Spray์™€ ํƒ€์‚ฌ์ œํ’ˆ (T์‚ฌ)์˜ ์„ฑ๋Šฅ ๋น„๊ต. A. RNase ์ œ๊ฑฐ: Removal reagent 4ฮผL์™€ ๋‹ค์–‘ํ•œ ์–‘์˜ RNase(1ฮผL)๋ฅผ ํ˜ผํ•ฉํ•˜์—ฌ ์‹ค์˜จ์—์„œ 5๋ถ„ ๋™์•ˆ ๋ฐ˜์‘ ํ•œ ํ›„, RNA 1ฮผL๋ฅผ ์ถ”๊ฐ€ํ•˜์—ฌ ์‹ค์˜จ์—์„œ 15๋ถ„ ๋” ๋ฐ˜์‘ํ•˜์˜€์Šต๋‹ˆ๋‹ค. ๊ทธ๋Ÿฐ ๋‹ค์Œ, ์ตœ์ข… ํ˜ผํ•ฉ๋ฌผ์„ formaldehyde ํฌํ•จํ•˜๋Š” agarose gel์—์„œ denaturation ๋ฐ ์ „๊ธฐ์˜๋™์„ ์ˆ˜ํ–‰ํ–ˆ์Šต๋‹ˆ๋‹ค. B. DNase ์ œ๊ฑฐ: Removal reagent 4ฮผL์™€ ๋‹ค์–‘ํ•œ ์–‘์˜ DNase(1ฮผL)๋ฅผ ํ˜ผํ•ฉํ•˜์—ฌ ์‹ค์˜จ์—์„œ 5๋ถ„ ๋™์•ˆ ๋ฐ˜์‘ํ•œ ํ›„, 1ฮผL์˜ 10X reaction buffer์™€ 1ฮผg์˜ DNA ๋ฐ nuclease-free water๋ฅผ ์ถ”๊ฐ€ํ•˜์—ฌ ์ด ๋ถ€ํ”ผ๋ฅผ 10ฮผL๋กœ ๋งž์ถ”๊ณ  ์‹ค์˜จ์—์„œ 15๋ถ„ ๋” ๋ฐ˜์‘ ํ•˜์˜€์Šต๋‹ˆ๋‹ค. ๊ทธ๋Ÿฐ ๋‹ค์Œ, ์ตœ์ข… ํ˜ผํ•ฉ๋ฌผ์„ agarose gel ์ „๊ธฐ์˜๋™์„ ์ˆ˜ํ–‰ํ–ˆ์Šต๋‹ˆ๋‹ค. C. DNA ์•ˆ์ •์„ฑ์— ๋Œ€ํ•œ removal reagent์˜ ์˜ํ–ฅ: removal reagent 4ฮผL์™€ 1ฮผL์˜ genomic DNA๋ฅผ ํ˜ผํ•ฉํ•˜์—ฌ ์‹ค์˜จ์—์„œ 15๋ถ„ ๋™์•ˆ ๋ฐ˜์‘ํ•œ ํ›„, agarose gel ์ „๊ธฐ์˜๋™์œผ๋กœ ๋ถ„์„ํ–ˆ์Šต๋‹ˆ๋‹ค. D. RNA ์•ˆ์ •์„ฑ์— ๋Œ€ํ•œ removal reagent์˜ ์˜ํ–ฅ: removal reagent 4ฮผL์™€ 1ฮผL์˜ RNA๋ฅผ ํ˜ผํ•ฉํ•˜์—ฌ ์‹ค์˜จ์—์„œ 15๋ถ„ ๋™์•ˆ ๋ฐ˜์‘ํ•œ ํ›„, formaldehyde๋ฅผ ํฌํ•จํ•˜๋Š” agarose gel์—์„œ denaturation ๋ฐ ์ „๊ธฐ์˜๋™์„ ์ˆ˜ํ–‰ํ•˜์˜€์Šต๋‹ˆ๋‹ค. ์ด ๋ฐ์ดํ„ฐ๋“ค์€ ์ฐธ๊ณ ์šฉ์ด๋ฉฐ, ์‹คํ—˜ ์กฐ๊ฑด์— ๋”ฐ๋ผ ๋‹ฌ๋ผ์งˆ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.

Application Notes

Nuc-Off Nucleases Removal Spray๋Š” ์‹คํ—˜ ํ™˜๊ฒฝ์—์„œ Nuclease๋ฅผ ํšจ์œจ์ ์œผ๋กœ ์ œ๊ฑฐํ•˜๊ธฐ ์œ„ํ•ด ์‚ฌ์šฉ๋˜๋Š” ์ œํ’ˆ์ž…๋‹ˆ๋‹ค.

Usage Statement

Research Use Only (RUO)

Specifications
SKU 1124604-CF
Application Notes Nuc-Off Nucleases Removal Spray๋Š” ์‹คํ—˜ ํ™˜๊ฒฝ์—์„œ Nuclease๋ฅผ ํšจ์œจ์ ์œผ๋กœ ์ œ๊ฑฐํ•˜๊ธฐ ์œ„ํ•ด ์‚ฌ์šฉ๋˜๋Š” ์ œํ’ˆ์ž…๋‹ˆ๋‹ค.
Storage 15-25ยฐC
Usage Statement Research Use Only (RUO)
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