Deoxyribonuclease I
Application Notes
DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Marker | Cell lysis | Enzymes | Inhibitors | Substrates
| SKU | 0219006210 |
| Alternate Names | DNase I;Deoxyribonucleate 5′-oligonucleotido-hydrolase; Deoxyribonuclease A |
| Application Notes | DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis. |
| Base Catalog Number | 19006210 |
| CAS # | 9003-98-9 |
| EC Number | 232-667-0 |
| Extinction Coefficient | 11.1 at 280 (Lit.) |
| Format | Powder |
| Pack Size | 10 MU |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| Preparation Method | Pancreatic deoxyribonuclease is unusually sensitive to physical denaturation by shaking. Mixing should be done by gentle inversion. Dissolve the standard in 1.0 mL of reagent grade water. Dilute further to a concentration of 20-60 u/mL. All dilutions are made in reagent grade water. |
| RTECS Number | RF0750000 |
| Source | Bovine Pancreas |
| Specific Activity | >50,000 Dornase u/mg solid |
| Typical Working Concentration | Dissolve at a concentration of 1 mg/mL. Dilute further to a concentration of 20-60 u/mL immediately before the assay. |
| Unit Definition | Amount of enzyme that causes the fall of 1.0 relative viscosity unit in a solution of highly polymerized DNA in 10 minutes at 30 °C from the intial relative viscosity of 4.0. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [190062] lot ID: R27363](https://www.mpbio.com/static/version1781253637/frontend/Mpbio/luma/en_US/images/document.png "MP COA [190062] lot ID: R27363")
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